Atmospheric cold plasma (ACP) is a novel non-thermal technique that provides the ability to denaturate enzymes without damaging main components. The first purpose of this research is to evaluate the impact of ACP produced via dielectric barrier discharge (DBD) on endogenous enzymatic activity and tilapia quality characteristics.How to cite this article: Mohamed EE, Younis ER, Mohamed EA. Impact of atmospheric cold plasma (ACP) on maintaining bolti fish (Tilapia nilotica) freshness and quality criteria during cold storing.