This study aimed to determine the effect of Up Hill and Down Hill exercises on increasing the 100 m run. The respondents of this study were 30 male athletes aged 16-17 years with a height of ±157.4 cm and a weight of ±56 kg. Samples were taken from extracurricular athletes who met the following criteria: they had been doing club training for 4-5 years, had done anatomical adaptation exercises before doing uphill and downhill exercises for 1 month, could carry out treatment for 16 meetings, and were in good health and not injured. Exclusion criteria are those that do not include requirements for inclusion. This research is an experimental study with a One Group Pretest-Posttest design as this research has a Pre-Test before being given treatment and a Post-Test after being given treatment. To test one's ability to run a 100 m sprint, one uses an instrument that measures one's ability to perform a 100 m sprint. An athletic track and a stopwatch are used as the tools in the 100-meter sprint ability test. The results showed that the average scores on the Pre-Test and Post-Test were ±11.80 and ±11.40, respectively. Based on the results of the analysis of the T-Test Paired Sample, the results of the Pre-Test and Post-Test were found at a significance of p>0.05 so it can be concluded that there are no significant effects on Up Hill and Down Hill exercises on increasing the 100-meter run.