Highly sensitive, rapid, accurate and precise synchronous fluorescence spectrofluorimetric method has been developed for simultaneous analysis of a mixture of amlodipine (AMD) and metoprolol (MET). The method relies on measuring the relative synchronous fluorescence intensity of both drugs at Δλ of 90 nm in acetate buffer solution at pH 5. The experimental parameters influencing the developed method were investigated and optimized. The method was linear over the ranges 0.2-2 μg/ml and 0.5-10 μg/ml for AMD and MET, respectively. The limits of detection were 50 ng/ml for AMD and 130 ng/ml for MET while the limits of quantitation were 150 ng/ml for AMD and 390 ng/ml for MET. The developed method was applied successfully for the determination of the two drugs in their co-formulated tablet. The mean percent recoveries were found to be 100.51 and 99.57 for AMD and MET, respectively.
Algae are one of the important sources of bio-diesel. Micro algae growth rate is directly affected by the sun light intensity and temperature. However, there is no available data in the literature concerning the environment temperature control to optimize the cultivate process of the algae in outdoor conditions. In the present study the outdoor continuous feedstock photo bioreactor has been designed and built to control the solar thermal radiation and its effect on the cultivation media temperature. Thos have been achieved using a new Air Bubble Generation (ABG) system. The used technique in the current study can enhance the temperature stability within the optimum range despite of the ambient conditions temperature. The results show that by increasing the air bubble generation rate the continuous photo bioreactor temperature distribution was enhanced along the reactor dimension. The optimum condition of the media PH is found to be 7.5, Na 2 CO 3 concentration of 5 g, the media width of 25 Cm, media height of 25 Cm and AWR (Air to Water Ratio by volume) of 150.
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