| Diseases caused by Pasteurella multocida (P. multocida) and Mycoplasma gallisepticum (M. gallisepticum) are of significant importance and induce great losses in poultry industry, so vaccination against diseases caused by both organisms is crucial. The present study was conducted to prepare inactivated vaccines from local strains of P. multocida and M. gallisepticum either alone or in combinations, evaluate these vaccines through measuring the immune response as well as detect the protection rates against the challenge with virulent P. multocida and M. gallisepticum strains. The prepared vaccine was evaluated by determination of the cellular immunity by heterophils/lymphocytes ratio (H/L) and evaluation of the humoral immunity by indirect haemagglutination (IHA) and haemagglutination inhibition (HI) tests. The potency of the vaccine was evaluated by the passive mouse protection and challenge tests against the challenge with the virulent strains of P. multocida (serotypes A and D) and M. gallisepticum (Eis3-10 strain). The results revealed that the combined inactivated P. multocida and M. gallisepticum vaccine adjuvanted with Montanide ISA70 induced high and long duration of antibody response and significant protection against the challenge with virulent strains of P. multocida and M. gallisepticum. In conclusion, the locally prepared combined inactivated P. multocida and M. gallisepticum vaccine elicited good cellular and humoral immune responses as well as high protection of chickens against both diseases.