This work was conducted to study the effect of Jojoba oil, lavender oil and their mixture on hypercholesterolemic rats. Thirty five male albino rats, weighing (150±10g), were divided into 7 groups (5 rats each) , 25 of them fed on1.5% cholesterol+0.2% bile salts to induce hypercholesterolemia The first main group fed on basal diet as a negative control group. One of the second hypercholesterolemic main group (5 rats) fed on basal diet as apositive control group , and the other hypercholesterolemic rats fed on basal diet and treated by Jojoba oil, lavender oil (2.5% and 5%) and mixture of them 2.5% for 4 weeks. At the end of the experiment serum samples were analyzed for the following parameters: Lipid profile; total cholesterol (T.C), total bilirubin, tri-glycerides (T.G), high density lipoprotein (HDLc), low density lipoprotein (LDLc), very low density lipoprotein (VLDLc) ; urea , creatinine , uric acid ; glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), alkaline phosphatase (ALP) and glucose. At the same time, the organs: Heart, kidney, liver, lungs and spleen were removed for weghting. The results indicated that treating rats with Jojoba oil, lavender oil and mixture of them decreased serum glucose, creatinine, uric acid, ALT, AST and ALP, as compared to the positive control group. It was concluded that the diet of Jojoba oil, lavender oil and mixture of them led to a significant decrease in lipid fractions ; (T.C),(T.G),(LDLc),(VLDLc) With increase of (HDL) , and these treatment are recommended to reduce the level of lipid profile for the patients with hypercholesterolemia.