Background: The hypoxia-inducible factor (HIF) hydroxylases (FIH and PHDs) regulate hypoxia sensing in animals.Results: FIH·HIF-α reacts faster with O2 than PHD2·HIF-α but slower than FIH·ankyrin complexes.Conclusion: The kinetics of catalysis by isolated FIH and PHD2 reflect their cellular activities.Significance: We provide a kinetic rationale for cellular observations that FIH activity is more hypoxia-tolerant than that of the PHDs.
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