The present study was carried out to determine the allelopathic effect of extraction of three herbs (Silybum marianum L. and Malva parviflora L. and Loliuim rigidum L. ) on three wheat cultivars (Rashid, Abo Ghrib and IPA99) on germination, growth and ear formation by classical research methods (laboratory and greenhouse experiments and molecular detection of GA3-oxase2 (TaGA3ox2-1) gene expression and its crucial role in wheat growth and Ta14S gene expression as a gene responsible for ear development. The study analyzed the influence of weed residues on the germination and growth of three wheat cultivars. According to the mean effect of aqueous weed extract on the cultivars, the laboratory experiments revealed a significant difference in all the characteristics studied. Keywords. Weeds, hybrid wheat cultivars, allelopathy, RT-PCR.