It has been proven that including probiotics in the diet of poultry will increase the quality of the meat and protein. Therefore, the purpose of this paper was to evaluate the administration of antibiotics and probiotics to broiler chickens and to determine the contribution of probiotics to the prevention of Salmonella typhimurium infection in Ross broiler hens. Three groups of one-day-old Ross broilers each contained: group1(G1-50 chicks), group2 (G2-100 chicks), and group3(G3 -100 chicks). (G1-50 chicks)negative management (non-infected and non-treated chicks). (G2-100 chicks) gentamycin and colistin sulphate were administered to chicks at the age of one who had S. typhimurium infections for five days at a dose of 0.5 gm/25 liters of drinking water. G3 third group (100 chicks) probiotics (Guardizen M) mixed probiotics concentrate 5.6 g (1x1010 cfu) of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus bulgaricus, and Bifidobacterium bifidum were given to chickens who had S. typhimurium infections. It was supplementary to the water for five days in a row. Each chick in the experimentally infected groups received an oral inoculation of 1 mL of saline suspension containing 109 CFU of S. typhimurium at the age of 6 days. After infection, the experiment's time frame was extended by three weeks. Take a swab for a Salmonella count to detect infection at 14, 21, and 28 days of age after the onset of symptoms. Results showed that group G3 had lower clinical symptoms and a lesser mortality level than group G2. The PM examination showed that the liver had hypertrophy, round borders, and white necrotic foci on its surface in addition to being discoloured. The bowel displayed enteritis with hemorrhagic patches, a bloody yolk sac that wasn't digested, petechial intestinal mucosal bleeding, and enteritis. In G2 treated with antibiotics, the S. typhimurium count was 4x107, 3x106, and 2x106 CFU in the second, third, and fourth weeks, respectively. Antibiotics were used to treat the other group, G3. The counts of S. typhimurium in the second, third, and fourth weeks were, respectively, 3.6x107, 2.3x106, and 1.1x106 CFU. In the meat of broilers nourished feed treated with probiotics and antibiotics, the percentage of protein content was 22,8% and 22,0%, respectively. The current analysis found that samples containing colistin sulphate had residue levels of 200 ppb that were higher than the permitted residue limits (150 ppb).