Background: Nephropathy is a significant cause of morbidity and mortality in patients with Diabetes Mellitus (DM). The condition is characterized by persistent albuminuria and may be decline in the Glomerular Filtration Rate (GFR). Urinary cyclophillin A has been proposed as a simple, accurate and rapid endogenous marker for DN. Aim of Study:To assess the value of urinary cyclophillin A anew marker of diabetic nephropathy.Subjects and Methods: Group I: 60 patients with type 2 diabetes mellitus which will be subdivided into: (A) 30 diabetic patients with diabetic nephropathy. (B) 30 diabetic patients without diabetic nephropathy. Group II: 30 healthy persons as a control group.Results: There was significance association between urinary cyclophillin A and serum creatinine, blood urea, 24 h protein collection and ACR.Urinary cyclophilin A found higher in diabetic nephropathy patients than diabetic patients without nephropathy so it is a new marker for diabetic nephropathy. Conclusion:The results of this study suggest that urinary cyclophillin A new marker for diabetic nephropathy.