The aim of this study was to evaluate the effect of 2% chlorhexidine (CHX) application during the bonding protocol on microshear bond strength of two adhesive systems, after storage in different media. Seventy-two human molars had their crowns cut in half and embedded in PVC cylinders with acrylic resin. The specimens were randomly divided into experimental groups (n=12) according to the adhesive system (Ambar and Single Bond 2), use of CHX in the bonding protocol, and time interval (24 h and 15 days) in the storage media (distilled water, mineral oil and 1% sodium hypochlorite -NaOCl). Adhesive systems were applied in accordance to manufacturers' recommendations, with or without the use of CHX, and resin composite (Z350 XT) cylinders were placed on the hybridized dentin. After photoactivation, the specimens were stored in distilled water, mineral oil and 1% NaOCl for 24 h and 15 days. Microshear bond strength was determined at a crosshead speed of 0.5 mm/min until fracture. The bond strength data were analyzed statistically by 4-way ANOVA and Tukey's test (α=5%). Use of CHX in the bonding protocol did not cause loss of bond strength in any of the evaluated situations, irrespective of time and storage medium. The storage medium had no influence on bond strength values after 15 days when the bond protocol without CHX application was used. However, the use of CHX in the protocol influenced negatively the bond strength values for Single Bond 2 after 15 days storage in distilled water and 1% NaOCl.

To prevent the rate of water absorption and degradation of exposed collagen and the resin matrix on the hybrid layers, the use of an additional layer of hydrophobic resin on all-in-one adhesives is one of the approaches to improve the bond strength and infiltration of monomers in demineralized dentin. Objectives:To compare the microshear bond strength of different self-etching adhesive systems, and to evaluate the effect of the application of a hydrophobic adhesive layer on all-in-one adhesive systems after a storage period of 48 h and 30 days in distilled water at 37oC. Material and methods: Bovine incisor crowns were polished to expose flat dentin surfaces. The crowns were randomly distributed into 14 groups (n = 12) according to the adhesive system [Clearfil SE Bond (CSEB), AdheSE (ASE), Adper Scotchbond SE (SSE), Adper Easy Bond (EB), and Go! (GO)], and storage time. In two groups (indicated as GO+B and EB+B), a layer of a hydrophobic adhesive was applied on all-in-one adhesive systems. After 48h and 30 days of storage in distilled water at 37oC, microshear bond strength was determined. The data were statistically analyzed by ANOVA and Tukey’s test (α = 5%). Results: After 48h, EB, EB+B, CSEB, and GO+B showed higher bond strength values. The application of a hydrophobic layer did not influence EB and increased GO bond strength values. After 30 days, CSEB, SSE, and EB+B showed the highest bond strength values. Comparing groups of all-in-one adhesives with and without a hydrophobic adhesive-resin layer, the bond strength values showed no significant difference. Conclusion:The application of a hydrophobic adhesive-resin layer increased bond strength values only at 48h. With respect to bond degradation over time, only EB showed a statistically significant decrease of bond strength after 30 days.

The success of implant-supported prostheses is related to the passive union between the prostheses’ connections and the implants, so it is necessary to obtain an accurate working cast. Objective: To evaluate the accuracy of the transfer impressions of implants performed with individual acrylic resin trays and conventional trays using polyvinyl siloxane. Material and methods: To carry out the transfer impressions, a master cast with four external hexagon implants parallel to each other was used. Five impressions were made with individual acrylic resin trays and five other impressions with modified conventional trays, opened in the region of the implants. The linear measurements between the implants were made with a digital caliper with an accuracy of 0.01 mm and the results were statistically evaluated �(alpha = 5%)�. Results: For the points AB and BC, the measurements obtained with the individual trays were statistically similar to the master cast. For the points CD and DA no statistical differences among the three groups were observed. Conclusion: Given the obtained results and the methodology used, it can be concluded that the impressions performed with individual trays presented higher accuracy compared to the ones obtained with conventional trays.