From yellow petals of Blue Mahoe, besides the known flavonoids gossypetin and gossypitrin, a new one gossypetin derivative was isolated from ethanolic extracts after Soxhlet extraction. Hence, in this study, we present a validated, sensitive and reliable NMR method for the simultaneous identification of flavonoids in this flower drug. Structure analyses of this flavonoid, revealed the identical glycoside moiety attached to a flavonol skeleton like gossypitrin, for which the structure of gossypetin-3'-O-β-glucoside was deduced from extensive NMR experiments.

The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants' composition has not been determined in detail so far. Hence, in this study, we present a validated, sensitive, reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode array detectors) method for the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, the structures of two compounds have been elucidated by LC-MS experiments after isolation. Structure analyses allow proposing the presence of gossypitrin (gossypetin-7-Oglucoside) or gossypetin-3'-O-glucoside, a quercetin derivative, possibly quercetin-3-O-glucoside and an unidentified compound with an impair number of m/z, probably an alkaloid.

A Fe(III)-gossypitrin complex was prepared with a metal to ligand molar ratio of 1:1; 2:1 and 3:2 by mixing stoichiometric amounts of gossypitrin and FeCl 3 . The isolated complex was characterized by visual observation, UV/Vis, IR and ESI-MS spectroscopy. In this study, we selected the major flavonoid glycoside present in the petals of the flowers of Talipariti elatum Sw. (Fryxell) Malvaceae that grows in Cuba, gossypitrin, to characterize the chelating activity of it chemical compound with FeCl 3 , to find alternative sources with lower side effects in asthmatic patients. A high chelating activity can be observed as a good source of new agents for asthmatic patients.

Abstract- Talipariti

Liquid chromatography (LC) coupled with UV detection and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the generation of chemical fingerprints and the identification of phenolic compounds in four solid samples extracted and isolated from the red petals of Talipariti elatum utilizing Soxhlet extraction with ethanol at 95 % during 20 hours. Three compounds were detected and two of them were identified as flavonoid glycosides while another one, is supposed to be and alkaloid with impair molecular weight. The compound with m/z of 464 after MS/MS analyses in both positive and negative ion mode is suggested to be Isoquercitrin. The mentioned compound has been identified in T. elatum for the first time in Cuban and Martinican flowers.