Campylobacteriosis is an infectious, zoonotic infection characterized by offspring and infertility, leading to economic losses in cattle and sheep breeding. In this study, we aimed to investigate the incidence of cattle and sheep abortion in Kars region in terms of Campylobacteriosis and evaluate the results by PCR, immunohistochemical, histopathological and microbiological methods. In this context, liver and lung tissue examples and abomasum contents of 444 abort cases brought from Kars Center and districts to Kafkas University Faculty of Veterinary Medicine Pathology Department between 2013-2019 years were examined. Tissue examples from animals were fixed in % 10 buffered formaldehyde solutions. After routine procedures, paraffin blocks were prepared and sections with a thickness of 5 μm were taken for Hematoxylin & Eosin staining and 4 μm were taken for immunohistochemical staining. Sections were examined under light microscope to determine histopathologic changes. Organs belonging to aborted fetuses and abomasum contents were inoculated into the Preston Campylobacter Enrichment Broth containing microbial study selective supplement, and then enriched by pre-enrichment and then passed through Preston Campylobacter Selective Agar. Cultures in which the culture was incubated after incubation were examined for colony morphology and microscopic appearance and Campylobacter spp. suspicious colonies were evaluated by biochemical tests. As a result of histopathologic studies, characteristically, 7 of 17 abortion cases with multifocal necrotic hepatitis pattern and yellow abomasum contents were blurred and clotted, PCR, immunhistochemical and microbiological methods detected as Campylobacter spp. positive towards the direction. As a result, we thought that Campylobacteriosis is an important place in the abortion cases from Kars region and should be taken into consideration in breeding. Kars İlinde Gözlenen Atık Vakalarında Kampilobakteriozisin Patolojik, İmmunohistokimyasal, PCR ve Mikrobiyolojik Yöntemler ile Araştırılması ÖZ Kampilobakteriyozis, sığır ve koyun yetiştiriciliğinde ekonomik kayıplara yol açan yavru atımı ve infertilite ile karakterize, bulaşıcı ve zoonotik bir infeksiyondur. Bu çalışmada, Kars yöresinde meydana gelen sığır ve koyun abort vakalarını Kampilobakteriyozis yönünden incelemek ve sonuçları PCR, immunohistokimyasal, histopatolojik ve mikrobiyolojik olarak değerlendirmek amaçlanmıştır. Bu kapsamda, 2013-2018 yılları arasında Kafkas Üniversitesi Veteriner Fakültesi Patoloji Anabilim Dalına Kars merkez ve ilçelerinden getirilen 444 adet atık vakasına ait karaciğer ve akciğer doku örnekleri ile abomazum içerikleri incelenmiştir. Hayvanlardan alınan doku örnekleri % 10'luk tamponlu formaldehit solüsyonunda tespit edildi. Rutin işlemlerin ardından hazırlanan parafin bloklardan, Hematoksilen Eozin boyaması için 5 μm, immunohistokimyasal boyama için kalınlığında 4 μm kesitler alındı. Histopatolojik değişikliklerin belirlenmesi amacıyla kesitler ışık mikroskobunda incelendi. Abort vakalarına ait organla...
In this study, we aimed to evaluate histopathologically the case of osteosarcoma detected in an Belgian Malinois race dog. The material of the study consisted of a 8.5-year-old Belgian Malinois race female dog weighing 30 kg. With the complaints of abdominal distention, difficulty in breathing, loss of appetite, fatigue, the dog brought to the Veterinary Faculty of Kafkas University Animal Hospital died on the way and was sent to the Department of Pathology for necropsy. In the follow-up, some of the tissue samples taken from the systemic necropsy animal were determined in the decalcified solution and another part in the buffered formaldehyde solution. Following routine procedures, 5 μm thick sections were taken from the prepared paraffin blocks for Hematoxylin Eosin staining. To determine the histopathological changes, the sections were examined by light microscope. In the light of the histopathological findings, osteosarcoma was diagnosed in the brain and lung metastatic foci.
ÖzetBu olgu sunumu ile iki buzağıda karşılaşılan ektopik böbrek olgularının klinik, histopatolojik bulguları ve operatif sağaltım sonuçlarının sunulması amaçlandı. Kafkas Üniversitesi Veteriner Fakültesi Cerrahi kliniğine getirilen buzağılardan ilkini kuyruk kısalığı ve arcus ischiadicus düzeyinde bir kitle bulunan Montafon ırkı, bir günlük dişi bir buzağı oluşturdu. Diğer olguyu ise perineal bölgede bir şişkinlik bulunan simental ırkı, dişi bir aylık bir buzağı oluşturdu. Her iki olguda da kitleler total olarak ekstirpe edildi ve histopatolojik olarak ektopik böbrek tanısı konuldu. İlk olgunun bir hafta sonra öldüğü diğer olgunun ise postoperatif 6. ayında sorunsuz bir şekilde yaşamına devam ettiği tespit edildi. Sonuç olarak yapılan literatür taramalarında ektopik böbrek olgularının perineal bölgede lokalize olduğuna dair kayıt bulunmamıştır. Bu yönüyle bakıldığında orijinal olarak görülen olguların literatüre katkı sağlayacağı söylenebilir. Anahtar sözcükler: Ektopik böbrek, Buzağı Case of Ectopic Kidney in Two Calves SummaryThe purpose of this report is to present the results of clinical and histopathological results and surgical treatment of ectopic kidney encountered in two calves. The first was a one-day-old female Montafon calf brought to the Kafkas University School of Veterinary Medicine Surgical Clinic because of a mass at the arcus ischiadicus and the fact that its tail was malformed. The other case was a onemonth-old female Simmental calf. It had swelling in the same area. After the masses were completely extirpated in both cases, they were evaluated histopathologically and ectopic kidney was identified. The first calf died one week later. The other calf was determined to be alive and well six months after the surgery. In conclusion, no record was found of ectopic kidney cases in the perineal region during the literature review. When viewed in this light, these original cases could be seen as a contribution to the literature.
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