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In a previous paper (Loebel, Shorr and Richardson, 1933) a survey was made of the metabolic conditions which favor the survival and development of the tubercle bacillus. In the present study we wish to examine the environmental features which interfere with its survival and development. The factors of "starvation," pH change, and anaerobic exposure will be considered, as well as the interaction of the cells of a tubercle with the invading organism. In general, the methods were the same described in the earlier paper. In order to prevent repetition, only the deviations from our usual technique will be discussed in the experiments to follow. SECTION I. DEPRIVATION OF FOODSTUFF ' This study is part of a group investigation in co6peration with the Research Board of the National Tuberculosis Association.