Purpose: This study was conducted to evaluate internalized homophobia and depression levels in LGBT individuals. Design and Methods: This descriptive study, conducted between June and October 2018, included 110 LGBT individuals. Findings: The mean Beck Depression Inventory total score of LGBT individuals was 3.14 ± 4.84. The mean total score of the Internalized Homophobia Scale was 15.5 ± 8.09. It was determined that there was a positive but weak relationship between the mean scores of internalized homophobia of the LGBT individuals and the mean score of depression, and internalized homophobia made a statistically significant contribution to depression. Practice Implications: It is considered that the presence of internalized homophobia should be taken into consideration in the prevention of depression in LGBT individuals or in investigating of existing depressive factors in LGBT individuals.