There has been no report on the prevalence of Campylobacter spp. in farm animals in Mongolia. To uncover the prevalence of Campylobacter spp. in chickens in Mongolia and their antimicrobial resistance, in this study, we isolated and characterized Campylobacter spp. from chickens in Mongolia. We collected 71 cloacal swabs of chickens from 5 farms including 4 layer farms and one broiler farm near Ulaanbaatar city and isolated 25 Campylobacter jejuni and 6 Campylobacter coli isolates. All isolates were resistant to tetracycline, and 3 C. coli isolates were resistant to erythromycin. The C. coli isolates possessed either the erm (B) gene or nucleotide substitution at nt 2,075 of 23S rDNA, both of which are known to be associated with erythromycin resistance. Sixteen of the 31 C. jejuni/C. coli isolates (51.6%) were resistant to nalidixic acid and fluoroquinolones. All the fluoroquinolone-resistant isolates possessed amino acid substitution from threonine to isoleucine at codon 86 (nucleotide substitution: A C A to A T A). Multilocus sequence typing and phylogenetic analyses showed a variation in C. jejuni / C. coli in chickens in Mongolia. In addition, some of the C. jejuni isolates seemed to be phylogenetically close to isolates in Asian and Oceanian countries. This is the first report on the characterization of antimicrobial resistance of Campylobacter spp. in farm animals in Mongolia and is valuable for implementation of measures for a prudent use of antimicrobials in farm animals.
We have done laboratory-based analyses of highly contagious and infectious swine diseases commonly occurring in household and pig farms on 200 swine serum samples collected from Bayanzurkh, Songinokhairkhan, Nalaikh, Khan-Uul district of Ulaanbaatar city, to establish the rate of infection, to gather information on farming and bio-security, to further detect this infectious diseases, for the purpose of risk assessment and preventive research. According to ELISA test results Classical swine fever 27 (13.5%), Parvovirus 50 (25%), Influenza 16 (8%), Circovirus 112 (56%) samples had shown positive results. Presence of classical swine fever antibody in 27/200 samples are reaction of vaccine immunization. Also antibody presence of Swine parvovirus, Influenza, circovirus in samples indicates necessity of make it clear that whether symptomless cases around farms. But antibody absence of porcine food and mouth disease, porcine reproductive and respiratory syndrome virus, swine mycoplasmosis transmissible gastro-enteritis, coronovirus, leptospirosis, brucellosis indicates such disease free in farms. Antibody absence of African swine fever done by surveillance this time shows no further infection however it was occurred in Mongolia early in 2019. Fundamental surveying is a key tool for monitoring health status of pig farm and formulating eradication program for swine infectious diseases as well as help farmers prevent economic losses by implementing prophylactic measures against sudden cases of such diseases. Thus we suggest that above mentioned surveillance study need to be continued as a tool for monitoring because of it is importance of keep pig farmers sustainable, furthermore crucial for nation`s economy. Улаанбаатар хотын ойролцоо үржүүлж буй гахайд зонхилон тохиолдох халдварт өвчнүүдийг тандан судалсан дүн Улаанбаатар хотын Баянзүрх, Налайх, Сонгинохайрхан, Хан-Уул зэрэг 4 дүүргийн өрхийн болон аж ахуйн гахайд зонхилон тохиолддог гоц болон халдварт өвчнүүдийн лабораторид суурилсан шинжилгээг хийх, халдварлалтын түвшинг тогтоох, аж ахуй эрхлэлт, биоаюулгүй байдлын талаар мэдээлэл цуглуулах, цаашид халдварт өвчнийг эрт илрүүлэх, эрсдэлийг үнэлэх, урьдчилан сэргийлэх судалгааны ажлын зорилгын хүрээнд нийт 200 дээжинд эсрэгбием илрүүлэх ЭЛИЗА урвалаар шинжилгээг хийхэд гахайн сонгомол мялзан 27 (13.5%), парвовирус 50 (25%), томуу 16 (8%), цирковирус 112 (56%) дээж эерэг дүн үзүүлэв. Гахайн сонгомол мялзан өвчний эсрэгбием (27/200) илэрсэн нь урьдчилан сэргийлэх зорилгоор тарьсан вакцины урвал юм. Гахайн парвовирус, гахайн томуу, гахайн цирковирус зэрэг өвчний вирусын эсрэгбием илэрч байгаа нь ил шинж тэмдэггүй, халдварын тархалт байгаа эсэхийг баталгаажуулах шаардлагатайг харуулж байна. Харин гахайн шүлхий, микоплазм, гахайн үржил, амьсгалын замын хам шинж, гахайн ходоод гэдэсний замын халдварт үрэвсэл, коронавирус, лептоспироз, бруцеллёз зэрэг өвчний эсрэгбием илрээгүй нь халдваргүйг харуулж байна. Гахайн африкийн мялзан өвчин Монгол оронд 2019 оны эхээр гарч байсан ч энэ удаагийн тандалтаар эсрэгбием илрээгүй нь өвчлөл дахиж гараагүй болохыг харуулж байна. Тандан судалгаа нь гахайн эрүүл мэндийг хянах, эрүүлжүүлэх хөтөлбөрийг боловсруулах гахайн аж ахуйд тохиолдох эдийн засгийн болзошгүй эрсдэлээс урьдчилан хянахад чухал ач холбогдолтой. Тиймээс энэхүү суурь судалгааг жил бүр тогтмол хийх нь гахайн аж ахуйг тогтвортой байлгах төдийгүй улс орны эдийн засагт чухал ач холбогдолтой тул гахайн эрүүл мэндийг хянах зорилгоор үргэлжлүүлэн хийж байх шаардлагатай байна. Түлхүүр үг: Гахайн халдварт өвчнүүд, ЭЛИЗА, тандан судалгаа, эсрэгбием
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