http://www.eje.cz altitudinal transects (Bubliy & Loeschcke, 2005). Even so, they show similar environmental patterns especially in climatic factors like temperature. In this way, altitudes are thought to mirror latitudes (Klepsatel et al., 2014) and thus similar clinal patterns are expected along altitudinal transects. Besides these differences and similarities, altitudinal transects occur over relatively short geographical scales, which may allow a relatively high gene fl ow between locations compared to latitudes and provide an opportunity to test the possibility of local adaptation in spite of gene fl ow (Keller et al., 2013).Drosophila melanogaster is a cosmopolitan species, which originated from sub-Saharan Africa and colonized Eurasia, America and Australia (David & Capy, 1988) and is the main organism used for investigating the adaptive basis of clines (Fabian et al., 2015). Drosophila, especially Drosophila melanogaster, are well studied in terms of latitudinal variation in traits including body size, abdominal pigmentation, ovariole number, resistance to starvation and desiccation, tolerance of heat and cold etc.