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Poly(A)‐containing RNA from polyploid uterine epithelial cells of Ascaris lumbricoides has been isolated by poly(U)‐Sepharose chromatography. The bulk of poly(A)‐containing RNA migrates as 18‐S RNA in formamide/polyacrylamide gels. In a cell‐free wheat germ system, this RNA directs the synthesis of a polypeptide with identical migration behavior in dodecylsulphate/urea/polyacrylamide gels as the polypeptide isolated from the proteinaceous eggshell. The two proteins reveal almost identical peptide patterns in fingerprint analysis. The authentic eggshell protein has been identified as a glycoprotein with a molecular weight of about 10 000, as determined by dodecylsulphate/polyacrylamide gel electrophoresis. The apparent discrepancy between mRNA length and the required coding length for the protein is discussed.