The saponin extracts of the aerial part Aloe vera Linn were examined for antibacterial activity in vitro against Streptococcus sanguinis. Streptococcus sanguinis is a gram-positive bacteria mostly found in dental plaque. Mechanical plaque control by brushing is the most effective method for controlling plaque and also gingivitis. Saponins Aloe vera L., natural materials for the treatment and prevention of disease is a strong surface active compounds, considered as one of the toothpaste composition and more acceptable by the body. Saponins or SLS induce to Streptococcus sanguinis bacteria and the minimal bactericidal concentrations determined by dilution method. TEM is used to evaluate the essential cellular function of Streptococcus sanguinis inhibited by the primary saponin-target interaction, whether they induce cell death (bactericidal drugs) or merely inhibit cell growth (bacteriostatic drugs) The variation morphological changes of bacterial cell, which is attributed to a whole cell wall with opaque cytoplasmic, cell wall separated from plasma membrane, plasma membrane rupture and event the cell wall peeled off revealed on the assays. Saponin Aloe vera L. extract was active against Streptococcus sanguinis with minimal inhibitory concentration value of 3.125%. The control group SLS killed Streptococcus sanguinis with minimal inhibitory concentration value of 0.78% in all repetitions. Saponin fraction aloe vera L. can inhibit the growth of Streptococcus sanguinis at concentration of at least 3.125%.

Primary school is a very strategic place for the prevention of dental and oral diseases. Delivering counseling material in learning oral health requires media. One of the media in dental and oral health education is through counseling using audio media. This audio media contains messages about dental health that are played at school every morning and can be exposed and heard every day so elementary school children can behave well in dental health. This study aims to determine the effectiveness of audio media outreach programs to the knowledge and level of dental and mouth hygiene of students at State Primary School 26 Kalimas Tengah, Kubu Raya Regency in 2019. This study was a quasi-experimental study with a Pre-Post observation research design. The samples of this study were 68 people which were class III, IV and V. The results showed the average rank of knowledge index before counseling with audio media was 8.928 and p was 0.020 (p<0.05) with index of dental and oral hygiene level was 0,00 and p of 0,000 (p<0.05). Meanwhile, after counseling with audio media was 9,500 and p of 0.020 (p<0.05) with a level of dental and oral hygiene of 35.00 and p of 0,000 (p<0,05). The conclusion of this study is counseling with audio media is more effective in increasing knowledge which can improve oral and dental hygiene

The Saponin extracts of the aerial part Aloe vera linn were examined for antibacterial activity in vitro against Streptococcus sanguinis. Streptococcus sanguinis, is a gram-positive bacteria mostly found in dental plaque. Mechanical plaque control by brushing is the most effective method for controlling plaque and also gingivitis. Saponins Aloe vera L, natural materials for the treatment and prevention of disease is a strong surface active compounds, considered as, one of the toothpaste composition and more acceptable by the body. Saponins or SLS induce to Streptococcus sanguinis bacteria and the minimal bactericidal concentrations determined by dilution method. The TEM is used, to evaluate the essential cellular function of Streptococcus sanguinis inhibited by the primary saponin-target interaction, whether they induce cell death (bactericidal drugs) or merely inhibit cell growth (bacteriostatic drugs). The variation morphological changes of bacterial cell, which is attributed to a whole cell wall with opaque cytoplasmic, cell wall separated from plasma membrane, plasma membrane rupture and event the cell wall peeled off revealed on the assays. Saponin Aloe vera L., extract was active against Streptococcus sanguinis with minimal inhibitory concentration value of 3.125%. The control group SLS killed Streptococcus sanguinis with minimal inhibitory concentration value of 0.78% in all repetitions. Saponin fraction Aloe vera L. can inhibit the growth of Streptococcus sanguinis at concentration of at least 3.125%.

(Riset Kesehatan Dasar, 2013) Rata-rata karies gigi (DMF-T) di Kalimantan Barat adalah 6,2 persen, yang berarti rata-rata 6 dari 28 gigi yang dimiliki penduduk perlu diperbaiki. Fakta bahwa ada lebih banyak profesional kesehatan daripada yang optimal hanya memperburuk masalah. Menurut informasi dari Konsil Kedokteran Indonesia (KKI) tahun 2013, hanya ada 166 dokter gigi di Kalbar. Karena masih banyak orang yang belum paham dan tidak sadar akan pentingnya menjaga kesehatan gigi dan gusi, maka tingginya angka kerusakan gigi. Hasil riset (Antara Kalbar, 2017) dari 400 peserta pemeriksaan gratis yang digelar Formula di Kota Pontianak menunjukan 80 persen memang bermasalah pada gigi berlubang.Saat ini masyarakat selain masih ada yang tidak memperhatikan cara menyikat gigi dan waktu yang benar melakukan menyikat gigi yaitu pagi setelah sarapan dan malam sebelum tidur . Hasil penelitian ini menjadi dasar yang sangat penting untuk diterapkannya program Edukasi serta pencegahan karies email dengan Fissure sealant diberikan kepada Siswa SDN 21 Pontianak Utara agar nantinya ada perubahan prilaku menjadi lebih baik dalam menangani masalah kesehatan gigi dan mulut. Berdasarkan hasil identifikasi masalah ditemukan beberapa masalah yang dihadapi mitra yaitu (1) kurang nya pengetahuan yang dimiliki Siswa di SDN 21 Pontianak Utara terkait pencegahan karies dengan fissure sealant, (2) kurangnya edukasi tentang kesehatan gigi dan mulut khususnya pencegahan karies dengan fissure sealant yang harus diterima oleh Siswa SDN 21 Pontianak Utara, (3) Belum adanya media Flipchart untuk menggambarkan tentang pencegahan karies dengan fissure sealant . Kontribusi mendasar program ini adalah transfer ilmu pengetahuan dan cara meningkatkan perubahan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut serta mengedukasi siswa tentang pencegahan karies dengan fissure sealant . Kegiatan ini akan merubah pengetahuan dan perilaku anak dalam menjaga kesehatan gigi dan mulut. Program yang diusulkan juga untuk menanggulangi masalah kesehatan gigi dan mulut yaitu dengan melakukan Edukasi menggunakan Flipchart pencegahan karies dengan fissure sealant