permits the continuous neutralization of the gluconic acid formed. With an improved nutrient medium and an organism able to tolerate the amount of boron necessary, it is possible to ferment 20, 25, and 30 per cent solutions of glucose in half the time previously reported. Under these conditions and with a process made semicontinuous by re-use of the mycelium, it is possible to ferment 25 per cent solutions of glucose to gluconic acid every 24 hours. This method is applicable to gluconic acid fermentations by cer tain bacteria as well as by fungi.
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