Objective. Perianal fistulas of the perianal soft tissues are an important cause of morbidity and a significant portion of colorectal surgery. To our knowledge, there is no study evaluating YouTube videos pertaining perianal fistulas. In this study, we aimed to evaluate YouTube video contents on this topic. Methods. Whether the videos contained real images, animations or presentations, video duration, number of views, comments and likes, uploading date, and daily view were recorded. Reliability of the videos was assessed using the Quality Criteria for Consumer Health Information (DISCERN) scale and quality of the videos using the Global Quality Score (GQS). Results. A total of 100 YouTube videos regarding perianal fistulas were included in the study. Sixty-four (64%) videos were uploaded by healthcare professionals and 36 (36%) videos by nonprofessionals. The mean video length was calculated as 6.70 ± 8.00 minutes for all videos. The mean DISCERN score of all videos was found as 3.92 ± 0.81 and the mean GQS score as 3.97 ± 0.83 . According to the DISCERN score, most videos included (94%) were of good quality. There was an excellent agreement between the two surgeons in terms of the DISCERN and GQS scores. Conclusion. Most of the videos included in the study were uploaded by health-care professionals. On the other hand, the majority of the videos contained surgical management of perianal fistulas as it is the definitive treatment. Healthcare related content should be audited and filtered by YouTube with new and effective policies.