TWIST is a transcription factor involved in the embryogenesis, the development regulating Epithelial-Mesenchymal-Transition and cellular migration. TWIST protein is able to form homo and heterodimers and modifications on its amino acids sequence that can alter its interactions with other proteins and the binding to DNA. A TWIST over expression was observed in different cancer cells as breast, prostate, lung, uterus, liver and several tumor cell lines. This TWIST over expression has been correlated with cancer development and poor overall survival in patients with breast cancer, its action mechanism is decreasing Ecadherin expression. This transcription factor is considered a critical Epithelial-Mesenchymal-Transition inductor; it participates in angiogenesis development and stem cells phenotype formation. An increasing or decreasing effect on the TWIST expression could be caused by chemotherapy agents in several cell lines, changing the cellular fate. TWIST is an important transcription factor that can be used as a target protein for cancer treatment.