In this case, semen that handled by urethral catheterization was deposited into the deepest point of the vagina of an anesthetized queen. Intravaginal artificial insemination was applied on the 2nd day of onset of the oestrus and only single insemination was performed. Insemination time was determined by behavioural and cytological evaluations. Two healthy kittens were obtained 62 days after insemination. The case has important reports in terms of being the first artificial insemination performance in cats in Turkey, resulted in parturition.