Background: Food borne diseases has remained a major global public health issue with substantial morbidity and mortality associated with the consumption of contaminated food staffs. Parasitic infection is among the major disease especially in developing countries and sub-Saharan countries.Objective:-This study was to determine the prevalence of intestinal parasites and associated factors among food handlers working in food and drink establishments at Mizan Aman town southwest Ethiopia.Method:- A community based cross sectional study was conducted in Mizan Aman town. A total of 418 food handlers from randomly selected 209 foods and drink establishments were interviewed and finally stool samples were taken. Parasitological assessment was performed by qualified laboratory technologists using the physiological saline for wet mount and zinc sulphate and formol ether concentration techniques were used. Data were analyzed using statistical SPSS version 21. P ≤0.05 was taken as statistically significantResult: The overall prevalence of intestinal parasites in Mizan Aman town was 26.1% .work experience >10 years (AOR=0.52,95% CI ;2.430,5.012),the food handlers who did not trimmed their finger,(AOR =1.89,95% CI :1.01,3.960) and using common knife for cutting raw meat and other foods (AOR=0.57, 95% CI : 3.61,5.54) were factors associated with parasitic infections. Conclusion: The magnitude of intestinal parasite in mizan aman town is high and work experience, finger trimming status, and using common knife for cutting raw meat and other foods were factors associated with the parasitic infections