Species of the genus Thymus L. (Lamiaceae) are of great interest as medicinal plants, as well as in the extracts and food technology industries due to the richness in bioactive specialized metabolites. Specifically, Iberian endemic species such as Th. piperella L., have a wide range of traditional uses in this sense. However, the micropropagation and biosynthesis of high-added value compounds under in vitro culture conditions has not been

Thymus webbianus Rouy (Lamiaceae) is an endemic plant known from only two populations located in theNatural Park "Penyal d 'Ifac" (Calp), and in the Natural Park of Serra Gelada (Benidorm-Alfàs del Pi) in Spain. At the Spanish state level, it has been listed as "Critically Endangered", and "Vulnerable" in the Valencian Region. Moreover, its taxonomic position remains unclear, and further research on this species is needed. Therefore, this work was aimed to characterize the chemical composition in volatiles from four subpopulations located in the Natural Park "Penyal d'Ifach" through HS-SPME-GC-MS.