NM_000546.5 >94% of the coding regions Markers for MSI analysis BAT25, BAT26, NR21, NR24 and NR27 Genomic DNA was isolated from deparaffinized formalin-fixed paraffin-embedded tumor and normal tissue using standard procedures. Library preparation and sequencing using a single-molecule molecular inversion probe (smMIP)-based NextSeq 500 approach were performed as described previously, 1 with the listed gene panel. Using a separate smMIP panel, 99% of the coding region of SMAD4 (SMAD4 RefSeq NM_005359.5) was analyzed. *For detection of point mutations and amplification. † For amplification detection. MSI, microsatellite instability.