Duguetia lanceolata St. Hill is a species native to the Atlantic Forest. Its seeds contain substances that have a germination inhibiting effect. In view of the above, the present work aimed to evaluate the influence of gibberellic acid and mechanical scarification on germination and seedling development of the D. lanceolata. This work was developed at IFSULDEMINAS - Campus Muzambinho, MG, Brazil. The seeds were obtained from fruits collected from adult plants. The experimental design was in randomized blocks, in a 5x2 factorial scheme with three replications with eighteen plants per plot. The treatments consisted of different concentrations of gibberellic acid (0, 5, 10, 15 and 20 mg L-1) and mechanical scarification (presence and absence). Leaf number, stem diameter and germination percentage were evaluated. The data were subjected to analysis of variance, the significant difference between treatments being determined by the F test and later, the qualitative variables were analyzed by the Scott-Knott test, and the quantitative variables by regression, both at the 5% level. The gibberellic acid and scarification do not influence the stem diameter and the number of seedling leaves. There was a linear increase in the percentage of germination with increasing doses of gibberellic acid up to the concentration 20.0 mg L-1 when scarification did not occur (35.19%). Quadratic behavior occurred with gibberellin doses when the seeds undergo mechanical scarification with a maximum dosage of 10.0 mg L-1 and corresponding germination of 42.59%. Mechanical scarification promoted an increase in germination when GA3 was not applied, and at the dosage of 10.0 mg L-1.
Meloidogyne is a relevant plant-parasitic nematode that causes enormous damage. It is very challenging to control, and there are not many chemicals available on the market for that. As an alternative method of nematode control, biofumigation is increasingly gaining space. This research aimed to study the reaction of Xanthosoma sagittifolium to Meloidogyne enterolobii, M. incognita, and M. javanica and soil biofumigation with X. sagittifolium leaves for M. enterolobii control. The reaction test was performed in the populations 0 (control), 333, 999, 3,000, 9,000, 27,000 eggs and eventual juveniles. X. sagittifolium did not host the Meloidogyne species studied, even in a high population. X. sagittifolium leaves incorporated in soil at concentrations 0 (control), 0.45, 0.9, 1.8, 3.6 g were also studied to control M. enterolobii, and they were able to reduce galls and eggs. The number of galls and egg masses was reduced to a concentration of 1.8 g. In the maximum concentration, the number of galls was less than 15 galls, and the eggs were also reduced to less than 200 eggs. As these macerates emitted nematicidal volatile organic compounds (VOCs) against M. enterolobii, it reduced the infectivity and reproduction of nematodes.
One of the limiting factors in coffee production is the aureolated spot caused by the bacterium Pseudomonas syringae pv. garcae. This work aimed to evaluate different Bayfolan® copper concentrations in the growth of two isolates of P. syringae pv. garcae in vitro. P. syringae pv. garcae 157 and 59 isolates were used. Two experiments were carried out in a completely randomized design (CRD) with 7 treatments and 5 replications, in a total of 35 experimental units. Each experimental unit consisted of a Petri dish. For the installation of the experiment, Bayfolan® copper was added and homogenized into Kado 523 culture medium at concentrations of 0; 625; 1.250; 1.875; 2.500 and 3.000 ppm, and as positive control the product Kasumin® was used at 3.000 ppm concentration. Bayfolan® copper reduced the growth of the two isolates evaluated in vitro with increasing concentration. At concentrations of 2.500 and 3.000 ppm, Bayfolan® copper inhibited completely the growth of both isolates, similarly to the Kasumin® treatment. The research reveals that Bayfolan® copper is efficient to control P. syringae pv. garcae in vitro from concentrations of 2.500 ppm onwards.
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