Cancer is a devastating disease characterized by abnormal cell growth. Due to several safety issues associated with current cancer treatment, identification of new strategies for cancer therapy is considered necessary. Natural substances in plants and fruits have the potentials to be developed as anticancer drugs. One of them is Cayratia trifolia (C. trifolia). This study was aimed to determine the cytotoxic activity of stem and leaf ethanol extract of C. trifolia against zygote cells of sea urchin or Tripneustes gratilla (T. gratilla). Extraction of stem and leaf of C. trifolia was conducted using maceration method in ethanol solvent. Thin layer chromatography was performed to screen phytochemicals contained in the extract. Zygote of T. gratilla was used to conduct antimitotic activity test. We found that stem and leaf ethanol extract had IC 50 of 169.82 μg/ml and 208.92 μg/ml, respectively. In comparison with positive control of vincristine which had IC 50 value of 3.23x10 -3 μg/ml (very strong category), this extract showed moderate antimitotic activity. In conclusion, C. trifolia is potential to be developed as anticancer treatment.

Galing (Cayratia trifolia L.) merupakan salah satu tumbuhan liar yang mayoritas terdapat di Asia Tenggara, termasuk Indonesia. Galing secara empiris telah banyak digunakan untuk mengobati berbagai macam penyakit. Hampir semua bagian dari tumbuhan galing dapat dimanfaatkan sebagai obat tradisional. Beberapa penelitian telah dilakukan untuk membuktikan aktivitas farmakologis dari tumbuhan galing. Oleh karena itu, tinjauan ini bertujuan untuk menyajikan informasi mengenai tumbuhan galing, baik secara biologi, fitokimia maupun aktivitas farmakologisnya.Kata kunci: Herbal, galing, Cayratia trifolia, obat tradisional, farmakologi

Cancer is a devastating disease characterized by abnormal cell growth. Due to several safety issues associated with current cancer treatment, identification of new strategies for cancer therapy is considered necessary. Natural substances in plants and fruits have the potentials to be developed as anticancer drugs. One of them is Cayratia trifolia (C. trifolia). This study was aimed to determine the cytotoxic activity of stem and leaf ethanol extract of C. trifolia against zygote cells of sea urchin or Tripneustes gratilla (T. gratilla). Extraction of stem and leaf of C. trifolia was conducted using maceration method in ethanol solvent. Thin layer chromatography was performed to screen phytochemicals contained in the extract. Zygote of T. gratilla was used to conduct antimitotic activity test. We found that stem and leaf ethanol extract had IC 50 of 169.82 μg/ml and 208.92 μg/ml, respectively. In comparison with positive control of vincristine which had IC 50 value of 3.23x10-3 μg/ml (very strong category), this extract showed moderate antimitotic activity. In conclusion, C. trifolia is potential to be developed as anticancer treatment.