Figure S1. Impact of BHRF1 expression on the mitochondrial membrane potential (MMP) and on the scoring of mitoaggresomes. (A and B) BHRF1 does not change the MMP. HeLa cells were transfected for 24 h with the BHRF1-HA plasmid or EV. Treatment with CCCP (2 h at 10 µM) was used as positive control to induce the loss of MMP. (A) Confocal images. Scale bar: 10 µm. (B) To assess the loss of MMP, colocalization between TOMM20 and CMXRos mitochondrial markers was measured by the Manders split coefficient using the ImageJ software. As MitoTracker CMXRos dye accumulates only in the mitochondria with intact membrane potential, the decrease in TOMM20/CMXRos colocalization level reflects a loss of MMP.