103 of helix I is important for DNA supercoiling but dispensable for binding to supercoiled DNA and 4WJs. We conclude that the B domain of HMG-1 can tolerate substitutions of a number of amino acid residues without abolishing the structurespecific recognition of 4WJs, whereas mutations of most of these residues severely impair the topoisomerase Imediated DNA supercoiling and change the sign of supercoiling from negative to positive.
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