The aim of the research is to improve the diagnosis of disturbances of cell energy in children with chronic nonspecific pulmonary diseases (CNPD) in the remission stage by the methods of detection of cytochemical changes of the energy status and recording the percentage of immune-competent cells of blood with the low membrane potential of mitochondria (MPM). 77 children were examined, including 66 (83%) with lung malformations, and 11 children (17%) with chronic nonspecific lung diseases as an outcome of acute/chronic lung diseases. The presence of energy deficient states was assessed by the level of activity of oxidative-reduction enzymes of succinate dehydrogenase, alpha-glycerophosphate dehydrogenase, lactic dehydrogenase in lymphocytes of the peripheral blood and percent ratio of lymphocytes with low MPM. It was found out that in 53.2% of the examined children even in the remission stage there were identified the signs of mitochondrial insufficiency when the parameters of metabolic activity of enzymes in lymphocytes were significantly lower than the norm. The cluster analysis of the obtained data allowed identifying the parameters typical for energy deficient states of the organism of the child that demand the correction by energotropic medications.

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Performing an experiment in which electronically microscopically studied the nature of reactive changes in the structural thin section of the spinal cord, as well as their dynamics during transcranial magnetic stimulation for 1 month after experimental neuromesis and after compression-ischemic neuropathy of the sciatic nerve. The reported development of compensatory-restorative processes in neurons, glial cells and the microvasculature of the lumbar spinal cord in rats that receive treatment with transcranial magnetic stimulation has been established. It was shown, that in all groups of rats changes in the structures of the lumbar thickening of the rat spinal cord developed in the form of depletion of the cytoplasm, destruction of organelles, changes in the nuclei and development of apoptosis of neurons and glial cells, destruction of the membranes and axial cylinders of myelin fibers. Moreover, these changes are more pronounced in groups after experimental neuromesis. However, in groups of rats, both after compression-ischemic neuropathy and after experimental neuromesis after treatment with transcranial magnetic stimulation, there were signs of the development of recovery processes in the form of intracellular repair of neurons, proliferation of oligodendrocytes, restoration of the structure of myelin fibers and capillaries, and the absence of free red blood cells in the extracellular space. The obtained morphological data confirm the effectiveness of treatment of transcranial magnetic stimulation of injuries of the peripheral nervous system in relation to neurons, glial cells, myelin and non-myelin fibers of the spinal cord.

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