The results of studies on assessing the influence of various types of fertilizers and growth regulators on the yield and quality indicators of grain varieties of winter barley on ordinary chernozem in the south of Russia are presented. According to the results of the experiments, it was found out that in the ecological test of varieties of winter barley, the yield of the standard variety Erema averaged 6.90 t / ha in the experiment. Among the studied varieties, only the Krasnodar variety Toma significantly and reliably exceeded the standard in terms of yield, having formed 7.62 t / ha. Assessment of the influence of organomineral, complex and micronutrient fertilizers, as well as a growth regulator on the yield and grain quality of winter barley varieties KA-12 showed that the yield on the control plots was practically the same and amounted to 7.12 and 7.10 t / ha, respectively. Treatment of seeds and vegetative plants with the studied preparations provided an increase in yield in comparison with controls. The greatest increase in yield was shown by the treatment of seeds and plants with micronutrient fertilizer Rexolin ABC - 1.38 and 1.58 t / ha, respectively. Similar results were obtained for the Erema variety. The treatments also contributed to the improvement of the quality indicators of winter barley grain.