Diseases that can be caused by environmental factors one of which is tuberculosis (TB). TB is an infectious disease that most often affects the lung tissue by Mycobacterium tuberculosis. Based on the observation of the condition of the settlement in the district Mulyorejo very crowded and poor sanitation, it may result in environmentally based disease is pulmonary tuberculosis (TB). This study was an observational study, the design of the study is a case-control study. Analysis of the data used are seeing the value OR. The sample was taken by purposive sampling with a sample size of 5 houses pulmonary tuberculosis patients and 10 home instead of pulmonary tuberculosis patients. The collection of data by observation sheet, physical measurements of air quality, and air sampling using Haz-Dust EPAM-5000 models, then dust samples were sent to the Institute of Tropical Disease Airlangga University for examination Mycobacterium tuberculosis bacteria in the air by PCR Aerosol. The results showed Mycobacterium tuberculosis in the air is a risk factor for pulmonary tuberculosis (OR = 2.667) and the physical condition of the house is a risk factor for pulmonary tuberculosis (OR = 2.667). People should improve the physical condition of the house, particularly the addition of mechanical ventilation to facilitate air circulation. For a house whose walls are attached to the neighboring wall can make the air vent on the roof, for further research using more samples to determine the factors that cause Mycobacterium tuberculosis in the home of respondents both patients and non-sufferers.Keywords:physical condition of the home, lung tuberculosis (TB), Mycobacterium tuberculosis