Plant cells can undergo regulated cell death in response to exogenous factors (often in a stress context), but also as regular element of development (often regulated by phytohormones). The cellular aspects of these death responses differ, which implies that the early signalling must be different. We use cytokinin-induced programmed cell death as paradigm to get insight into the role of the cytoskeleton for the regulation of developmentally induced cell death, using tobacco BY-2 cells as experimental model. We show that this PCD in response to kinetin correlates with an arrest of the cell cycle, a deregulation of DNA replication, a loss of plasma membrane integrity, a subsequent permeabilisation of the nuclear envelope, an increase of cytosolic calcium correlated with calcium depletion in the culture medium, an increase of callose deposition and the loss of microtubule and actin integrity. We discuss these findings in the context of a working model, where kinetin, mediated by calcium, causes the breakdown of the cytoskeleton, which, either by release of executing proteins or by mitotic catastrophe, will result in PCD.
Zarówno w świetle Biblii i Koranu, jak i derywatów od pie. gen- (zob. łac. gentis ‘rodzaj, gatunek’) nazwa żona oznaczała ‘ta, która rodzi, współtworzy’. Do polszczyzny, razem z całym słownictwem chrześcijańskim, przeniknęła z niemieckiego przez czeski. W niemieckim nabrała dodatkowego znaczenie ‘ta, która jest poślubiona’. Część dawnych nazw, np. mężyca, zanikła.
The evolution of semantic field of marriageBased on historical and linguistic material, the article presents the evolution of marriage as institution in Poland from the pagan age until the present day, focusing above all on the function of gender roles ascribed to man and woman in this context. A recent rapid increase in changes can be observed in the Polish language. Some elements of vocabulary semantically associated with marriage have fallen into oblivion; some have been preserved as so-called “historical archaisms”, i.e. words commonly known yet not used; others have changed their meaning or emotional tone (sometimes both). Words of unaltered meaning (alterations in the form were left unexamined as they are not significant for the problems discussed in the article) are relatively few. A study of their environmental and generational frequency both in the past and at present should be conducted, impossible to be included in the article. The article is limited to the presentation of facts, leaving the assessment thereof to the readers.
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