This study was done to determine the degree of metals and heavy metals in some bee pollen sample as biological indicator for environmental pollution. Sample were collected from industrial, urban and agricultural areas of Jordan in year 2017. Eight metals (As, Cd, Cu, Mg, Pb, Ni, Se, and Zn) continents analyzed by using Inductively Coupled Plasma Optical Emission Spectrum, (ICP-OES).Results were statistically interpreted by using ANOVA analysis. Metals content in bee pollen from Jordan and China were determined within the following ranges (minimum–maximum mg/kg); Cu (copper): 11.338–0.032, Zn (Zinc): 77.022–25.24, Ni (Nickel): 2.839 to <0.01, Se (Selenium): 3.03 to <0.04, Mg (Magnesium): 1575.19–641.388, Pb (Lead): 2.567 to <0.03, Cd (Cadmium): <0.005, As (Arsenic): <0.02. The results showed that there were no statistically significant differences among metals in the bee pollen. It has also found that bee pollen produced in Jordan may be a good source of some trace elements like Se and Mg and can be used as an environmental indicator and for quality control.

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An accurate and simple quantitative method for determining levodopa and methyldopa is proposed. Cerium(IV) has been successively used to react with drugs in 2 M H2SO4 at 80° C. The absorbance of colored products was measured at 510 and 550 nm for levodopa and methyldopa, respectively. The method has been extended to the determination of these drugs in dosage forms. The results have been statistically compared with those obtained by the reference method.

A rapid, simple, and sensitive RP-HPLC analytical method was developed for the simultaneous determination of triclabendazole and ivermectin in combination using a C18 RP column. The mobile phase was acetonitrilemethanolwateracetic acid (56 36 7.5 0.5, v/v/v/v) at a pH of 4.35 and flow rate of 1.0 mL/min. A 245 nm UV detection wavelength was used. Complete validation, including linearity, accuracy, recovery, LOD, LOQ, precision, robustness, stability, and peak purity, was performed. The calibration curve was linear over the range 50.09150.26 g/mL for triclabendazole with r = 0.9999 and 27.0181.02 g/mL for ivermectin with r = 0.9999. Calculated LOD and LOQ for triclabendazole were 0.03 and 0.08 g/mL, respectively, and for ivermectin 0.07 and 0.20 g/mL, respectively. The intraday precision obtained was 98.71 with RSD of 0.87 for triclabendazole and 100.79 with RSD 0.73 for ivermectin. The interday precision obtained was 99.51 with RSD of 0.35 for triclabendazole and 100.55 with RSD of 0.59 for ivermectin. Robustness was also studied, and there was no significant variation of the system suitability of the analytical method with small changes in experimental parameters.