No abstract

A simple, rapid and sensitive method was developed and validated using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for determination of Bezafibrate (BEZ) in human plasma. The plasma samples were extracted by protein precipitation using bezafibrate-d4 as Internal Standard (IS). The chromatographic separation was performed on Sunfire C18, 3.5µ, 2.1x50mm with a mobile phase consisting of formic acid, water and acetonitrile at flow rate of 0.30 mL/min. BEZ was detected and identified by mass spectrometry with Electrospray Ionization (ESI) in negative ion and Single Ion Recording (SIR) mode. The method was linear in the range of 100-20000 ng/mL for BEZ. The lower limit of quantification was 100 ng/mL. This method could be applied to bioequivalence studies in human plasma samples.