No abstract

No abstract

A simple new experiment that uses UV-vis spectrophotometry to introduce undergraduate chemistry students to multicomponent analysis is presented and a method for the simultaneous determination of three vitamins using derivative spectrophotometry (zero-crossing method) is described. The methodology is simple and easy to apply and allows the determination of folic acid, pyridoxine, and thiamine over the concentration ranges 1.02-14.28, 1.00-16.00, and 6.00-20.00 μg mL-1, respectively. The resulting errors were nearly always less than 5%.