In 12 female subjects participating in a 100-km run, the concentrations of cholesterol and triglycerides as well as those of HDL components (total cholesterol, cholesteryl ester, unesterified cholesterol, phosphatidyl choline, apolipoprotein A-I, and apolipoprotein A-II) were measured before and after the run. The concentrations of HDL unesterified cholesterol, HDL phosphatidyl choline, and HDL apolipoprotein A-I of the participants of the 100-km run were higher than the corresponding values of a control group of female company employees, whereas there was no difference in triglycerides, total cholesterol. HDL cholesterol, and HDL apolipoprotein A-II. Values of triglycerides and total cholesterol ascertained after the run were clearly decreased when compared to pre-exercise values (by 70% and 10%, respectively). Also, total protein content of the serum was marginally reduced after the run. Considering the reduction in protein level. HDL cholesterol and HDL apolipoprotein A-I values were raised after the run (P less than 0.01, P less than 0.05, respectively) while HDL phosphatidyl choline values decreased irrespective of the reduction in total protein (P less than 0.01). The quotients HDL cholesterol / total cholesterol as well as HDL cholesterol / HDL phosphatidyl choline were clearly higher after the run than before (P less than 0.01). The HDL apolipoprotein A-II level did not change post-exercise. These results indicate a change in the structure of HDL following a 100-km run in female athletes.

An economical enzymatic assay for HDL phosphatidyl choline is described, äs adapted for the Cobas-Bio analyser (Hoffmann LaRoche). This method entails the enzymatic cleavage of phosphatidyl choline by phospholipase C from B.cereus, hydrolysis of phosphoryl choline and enzymatic determination of choline with choline oxidase by an enzymatic colour test. This method provides consistent values and is, by comparison to the enzymatic UV method (assaying choline with choline kinase in an optical test procedure), simpler to perform, more precise, and less expensive. Eine rationelle Methode zur Bestimmung von HDL-Phosphatidylcholin Zusammenfassung: Es wird eine rationelle enzymatische Bestimmungsmethode von HDL-Phosphatidylcholin beschrieben, die an den Analysator Cobas-Bio (Hoffmann LaRoche) adaptiert wurde. Das Prinzip dieser Methode besteht in der enzymatischen Spaltung von Phosphatidylcholin mittels Phospholipase C aus B.cereus und der enzymatischen Bestimmung von Cholin mittels Cholinoxidase in einem enzymatischen Farbtest nach Hydrolyse von Phosphorylcholin. Das Verfahren liefert im Vergleich zur enzymatischen UV-Methode (Messung* von Cholin mittels Cholinkinase im optischen Test) übereinstimmende Werte und ist im Vergleich zur enzymatischen UV-Methode einfacher durchführbar, präziser und billiger.

Summary:A turbidimetric assay for the quantification of apolipoprotein A-II in serum is described. Its adaptation for the Cobas Bio Analyser (Hoffmann La Röche) is reported.Regression analysis of apolipoprotein A-II values measured by turbidimetry revealed a good measure of agreement with the data obtained by radial immunodiffusion (RID) (i\= 0.90, y = 0.97x + 0.021, n = 100) äs well äs with data obtained by immunonephelometry (r = 0.838, y = 0.925x + 0.029, n = 34). A Variation coefficient of 3.2% (n = 18) was found in relation to the precision in the series and a Variation coefficient of 3.5% (n = 36) in relation to day to day precision. Turbidimetrische Bestimmung von Apolipoprotein A-IIZusammenfassung: Es wird ein turbidimetrisches Verfahren zur Bestimmung von Apolipoprotein A-II im Serum beschrieben, das an den Analysator Cobas Bio (Hoffmann La Röche) adaptiert wurde.Die Regressionsanalyse der mittels Turbidimetrie ermittelten Apolipoprotein A-II-Werte ergab eine gute Übereinstimmung mit Apolipoprotein A-II-Werten, die mittels radialer Immundiffüsion (RID) (r = 0,90, y = 0,97x + 0,021, = 100) bzw. mittels Immuntiephelometrie bestimmt wurden (r = 0,838, y = 0,925x + 0,029, n = 34). Der Variationskoeffizient in der Serie betrug 3,2% (n = 18) und der Variationskoeffizient von Tag zu Tag 3,5% (n = 36).

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