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Highlights-Following a previously published field investigation, this study aims to characterize tritium effects on fish health under controlled conditions in a laboratory setting.-Fathead minnows were exposed to tritium activity concentrations up to 180,000 Bq/L.-At the highest levels of exposure, tritium increased DNA damage and modulated the immune responses.-Other markers were affected, including the neural system, oxidative stress and fatty acid composition.-No effects are reported on the measured health indices and anti-oxidant activities.

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