Background: The current research aimed to explore secondary metabolites and antibacterial activity against bacterial agents associated with bovine mastitis from extracts prepared from the peel of fruits of Punica granatum L.. Mastitis is an inflammation in the mammary gland mainly due to infection of bacteria and can be presented clinically or subclinically. The infectious bovine mastitis disease is associated with the reduction and changes in the composition of the milk, as well as in the early disposal of the animal. Objective: We describe the bactericidal activity of ethanolic extracts of the fruit peel of P. granatum, against etiological agents of bovine mastitis (Escherichia coli, Staphylococcus aureus and Streptococcus agalactiae). Method: The bactericidal activity was evaluated according to the standards of the European Committee for Antimicrobial Susceptibility Testing and by the broth microdilution methodology according to the standards of the Clinical and Laboratory Standards Institute. The fruit peel extracts of P. granatum were prepared with ethanol solvent (90%). Results: The best results of total inhibition of bacterial growth of the three strains tested were obtained with the concentrations of 75mg/mL of the extract after 1 and 3 hours of treatment. Cell membrane integrity analysis by flow cytometry showed that 1 h of contact there was loss of integrity of the wall and cell membranes. Conclusion: We can suggest that the ethanolic extract of the fruit peel of P. granatum can be an important natural coadjutant compound in the treatment of mastitis as well as being used in the formulation of sanitizers.

Background: Pectolinarigenin is a natural flavonoid compound having molecular formula C17H14O6 and molecular weight 314.28, found in different plants such as vegetables, medicinal herbs, and fruits. Eupatorium odoratum, Cirsium chanroenicum, Cirsium setidens, Clerodendrum phlomidis, Cirsium japonlcum, Chromolaena odorata and Trollius chinensis are some of the best natural source of pectolinarigenin. Objective: The purpose of this review paper is to summarize all the pharmacological properties of pectolinarigenin on cancer and other disorders, describe the mode of action and possible pathways for cellular level action. Method: Various databases have been searched to collect all the information of pectolinarigenin and presented in the form of scientific review. Results: Pectolinarigenin acts as an anticancer agent against various types of human malignancies such as lung cancer, melanoma, hepatocellular carcinoma and colorectal adenocarcinoma. It also has anti-inflammatory, anti-allergy, cytotoxic and hepatoprotective properties. Pectolinarigenin gained attention from researchers and clinicians due to their anticancer properties and in future it could be the best choice for cancer treatment. The present review initially highlights the current status of flavonoids and their pharmaceutical importance, role of pectolinarigenin in human disorders, and in later section, summarizes analytical techniques of pectolinarigenin as lead molecules. Conclusion: Conclusion: This review will support all the ongoing research of pectolinarigenin through out world to for their beneficial properties in all the scientific discipline. Other: Plants are valuable source of phytochemical which are mainly used as food material but more specifically as a medicine since very ancient time in the world. Flavonoids basically contain three rings and present in the fruits, vegetables, grains, wine and tea. Strawberries, broccoli, apple, berries, grapes, citrus fruit and lettuce are some of the best examples of rich source of flavonoidal compounds. Flavonoidal compounds have attracted many scientists due to their attractive colour and physiological properties. In addition to their role in nutrition, flavonoids possess many types of pharmacological activities including anti-viral, anti-bacterial, anti-cancer, anti-inflammatory, cardioprotective, anti-diabetic and anti-aging properties.