Background. The aim of this study was to analyze the concurrent validation between the resistance index (RI) obtained by Spectral Doppler (SD) and the vascular resistance (VR) calculated by quantifying pixel color intensity of the power Doppler (PD) signal. Methods. The brachial artery of 30 healthy participants (24.8 yrs; SD = 6.44 yrs) were evaluated with SD to automatically obtain RI and with PD to estimate de VR with de Pourcelot's formula from systolic and diastolic peaks. Three assessments were performed on each participant, obtaining a total of 90 ultrasound assessments of the brachial artery with their respective RI. Processing and analysis were performed ImageJ software were manually selected and extracted from the brachial artery PD images with the highest and lowest signal corresponding to peak systolic and end diastole for each patient. The mean pixel color of the image with the highest signal was considered as the peak systolic velocity and of the image with the lowest signal as the end-diastolic velocity. Results. A high correlation was found between RI and VR (r = 0.92; 95%CI = 0.88 to 0.95; p ≤ 0.001) so there is a very strong concurrent validity between the two measures, and they can be considered equivalents (common variability of 84%). Conclusions. This new method of analyzing DS by quantifying the color intensity of the PD signal pixel is a good predictor of RI and could be useful for VR analysis in musculoskeletal tissues where measurement of RI is complicated such in neovascularization in tendinopathies with multiple Doppler signals.