Despite the productivity, achieving long-term sustainability and maintaining plant biodiversity have become the pivotal goals in orchard floor management, especially along tree rows. Thus, the paradigm of eradicating weeds in the tree row using chemical herbicide or repeated soil tillage needs to be substituted with more sustainable alternatives. This study was conducted in two commercial apple and peach orchards in Marche region (Italy). Two integrated mechanical approaches, integrated mowing (mower and brush or disc) and integrated tillage (blade weeder and integrated mowing), were compared with the standard herbicide system in a 2-year trial. Weed species diversity, soil coverage, and weed biomass production, including, gas exchange parameters, trunk cross-sectional area (TCSA), fruit yield and quality were measured. Overall, both integrated practices demonstrated approximately 82%, 91% and 113% more species diversity, soil coverage, and weed biomass production, respectively, than herbicide systems. No significant differences were found in terms of tree gas exchange parameters, growth and fruit yield. However, a few fruit quality parameters such as fruit firmness, soluble solids content (SSC) and dry matter content responded positively to the integrated practices. These results suggest that the integrated mechanical approaches of weed management increased orchard biodiversity, and had no adverse effects on tree growth, fruit yield, and quality. The average costs per hectare associated with chemical weed control were 66.5% and 72% lower, respectively, compared to integrated tillage and integrated mowing. However, the government subsidies provided to the orchardists to encourage sustainable management practices were able to offset such additional costs.