Beetles of the genus Nitidula Fabricius are forensically important, and their adults and larvae have been found associated with human corpses and animal carcasses in many places of the world. The external morphology of the larvae of Nitidula carnaria (Schaller 1783) was examined by scanning electron microscopy (SEM) to provide a description enabling identification of this forensically important species. The ultrastructure of the head was examined, antennae, mandibles, epipharynx, maxillary and labial palpi, spiracles, thorax, legs, and abdominal segments (especially segments 9 and 10); the tegument was also emphasised in this examination. Several types of sensilla were observed on the maxillary and labial palpi, including sensilla basiconica, sensilla styloconica, and perhaps a different type of sensilla digitiformia. In abdominal segment 10, a sensilla campaniformia was observed. Two types of plates were noticed in the abdominal tegument. The characteristics described here can be used to identify this species. No other study of the ultrastructure of Nitidulidae larvae is available for comparison. This is the first report of N. carnaria in carcasses in Chile.
The objectives in the present work were to identify maize double haploids one generation after chromosome duplication through the evaluation of phenotypic characteristics in thr field, flow cytometry and molecular markers SSR. The seeds used in the present study were obtained from a cross between four simple hybrids (DKB393, GNS 3225, GNS 3264, GNS 3032) and the KEMS inducer of gymnogenetic haploidy, used as a male parent. Seeds from this crossing were selected according to the R-Navajo marker and those considered haploid, were submitted to two different chromosome duplication protocols. Plants that survived to the chromosome duplication protocols were acclimatized in greenhouse and later transplanted to the field. After self-fertilization of the DH0 plants, the DH1 seeds obtained were seeded in the field, divided into treatments according to the parental and duplication protocols. At the vegetative stage V4 of the DH1 seedlings, leaf tissue samples were collected to identify ploidy via flow cytometry and DNA analyzes using microsatellite markers. These results were confronted with the morphological characteristics of the future DH1 plants developed in the field, evaluated with the use of descriptive tools. Statistical analyzes were performed using the generalized linear modeling approach and the exploratory and inferential analyzes of datas, by the use of graphical resources, barplot and boxplot. For the analysis of variance, were used the Student-Newman-Keuls test, and the Pearson's correlations. It was not observed uniformity of phenotypic characteristics of plants subjected to duplication protocols in the field and the use of descriptive tools in the morphological analysis of adult maize plants must be done carefully to avoid the wrong classification of determined genotypes related to the ployd. Flow cytometry must be used as screening in the identification of possible DH´s and the molecular markers SSR can be used to prove the genetically inherited KEMS lineage and also to identify the double-haploid corn plants.
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