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The investigation was carried out to determine the susceptibility of ABO blood groups to malaria parasiteamia among residents of Awka, Awka South L.G.A, Anambra State Nigeria. The research revealed that out of 309 samples analyzed for ABO blood groups susceptibility for malaria in various classes, 265 (85.8%) was observed to have patent parasiteamia with malaria. Among the sexes, males recorded higher susceptibility than females, with blood group B recording highest (94.5%) susceptibility of infection than other blood groups.The ABO and Rhesus blood group phenotype sample were determined by agglutination method using commercially provided antisera suitable for the detection of blood rhesus positivity and negativity.

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Acute toxicity of butachlor, a chloroacetanilide herbicide (2-chloro-N-[2, 6-diethylphenyl] acetamide) to Tilapia zillii, was studied in a semi static bioassay. The 24, 48, 72 and 96 h LC 50 values (with 95% confidence limits) estimated by probit analysis were 3.13 (2.88 to 4.61), 1.93 (0.63 to 4.41), 1.27 (0.59 to 1.92) and 1.25 (0.60 to1.85) mgl-1 , respectively. There were significant differences (p<0.05) in the LC 50 values obtained at different exposure times and the safe levels estimated by different methods varied from 1.25×10-1 to 1.25×10-5 mgl-1. There were dose and time dependent increase in mortality rate due to exposure to the herbicide. Stress signs in form of hyperactivity, erratic swimming, skin discoloration, vigorous jerks of the body followed by exhaustion and death were observed. The 96 h LC 50 of 1.25 mgl-1 obtained indicate that the herbicide was toxic to T. zillii. Agricultural use of butachlor in the environment, especially, near water bodies, must be restricted to avoid the severe risk associated with the use of the pesticide.