No abstract

A total number of 1260 fertile eggs were randomly divided into seven treatments to investigate effect of in ovo-injection of ascorbic, folic acids and their combination at 14 th day of incubation on hatchability and subsequent growth performance of broiler chicks. The experimental design was as follow: 1-Eggs were without injection. 2-Eggs were injected with 0.1ml distiller water/ egg. 3-Eggs were injected with 0.1ml solution containing 6 µg ascorbic acid (AA) /egg. 4-Eggs were injected with 0.1ml solution containing 75 µg folic acid (FA) /egg. 5-Eggs were injected with 0.1ml solution containing 150 µg FA /egg. 6-Eggs were injected with 0.1ml solution containing 6 µg AA + 75µg FA/egg. 7-Eggs were injected with 0.1ml solution containing 6 µg AA+150µg FA /egg. Hatchability % was improved due to injection of AA 6 /egg and with combination between AA 6 and FA 150 /egg as compared to the control group. Also, in ovo injected with AA 6 , FA 75 , FA 150 and AA 6 +FA 75 recorded the best value of live body weight compared to the control group at 28 days of age. In ovo injection of AA, FA and their combination significantly decreased triglycerides levels in serum blood. Also, the level of HDL significantly improved due to in-ovo injected with AA 6 and FA 75 . Therefor, it could be mentioned that in-ovo injection of 6µg AA/egg and 6µg AA+150µg FA/egg in fertile eggs at 14 th day of incubation can be applied to improve the hatchability and subsequent growth performance from 1 to 28 day of age.