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An E. coli strain in which all known pathways of threonine catabolism were inactivated (Δtdh, ΔltaE, ΔAilvA, ΔtdcB, AyiaY) has been constructed. The possibility of an alternative pathway for the isoleucine synthesis by expressing heterologous citramalate synthase from Leptospira interrogans in an E. coli strain carrying the ΔilvA deletion was demonstrated. It was observed that the cimA overexpression has a negative effect on the threonine production. We developed a system for regulated gene expression based on the inducible promoter PLtetO and TetR repressor of the tetracycline operon. A threonine producing strain B-1201 in which the cimA gene is expressed under the control of the regulated promoter was constructed. A correlation of the threonine productivity and the expression level of the cimA gene was shown by culturing the B-1201 strain in fermenter. The optimal inductor content for the maximum threonine accumulation was also determined. Escherichia coli, strain, threonine, citramalate synthase This work was supported by the Ministry of Education and Science of the Russian Federation (project code RFMEFI61017X0011), and it was carried out using the equipment of the National Bio-Resource Center «All-Russian Collection of Industrial Microorganisms», NRC «Kurchatov Institute» - GOSNIIGENETIKA. The authors are grateful to Dr. I. V. Manukhov for providing the MG1655Z1strain and pZE21-lux plasmid, and for teaching techniques of bioluminescence measuring.

Abstract:In chronic stage of stroke, it is necessary to pay attention to the complex spatial movements training along with the traditional restoration of balance, strength of particular muscles, and paretic limb joints mobility. The aim of the study was to evaluate the effectiveness of robotic therapy in the recovery of upper limb function in the chronic stage of stroke. The study involved 52 patients with ischemic stroke in the middle cerebral artery. The patients were divided randomly into 2 groups. All patients (5 days/wk × 3 wk) got gymnastics by the standard technique, massage, laser, and pulsed currents therapy. Main group patients (n = 36) extra received complex spatial movements, speed, fluidity, precision and agility training by the robotic electromechanical device Multi Joint System (MJS) (40 minutes, 5 days/wk × 3 wk). Analysis of the results of the study showed a statistically significant difference in improving ROM of the elbow and shoulder joints, speed and accuracy of movement in the main group compared with the control. Hardware recovery of complex spatial upper limb movements in the chronic stage of stroke increases the functionality and independence of the patient's domestic skills.