Our data show that the addition of limited exercise to dipyridamole results in benefits during Tc-99m sestamibi imaging, increasing heart-to-liver activity ratio, preventing vasodilator-induced hypotension, and improving ECG sensitivity for the detection of ischemia. Furthermore, this protocol also provides an estimation of the patient's physical capacity and could be used as an alternative for patients undergoing dipyridamole infusion who are not completely unable to exercise.

Fatal vaccine-induced canine distemper virus inifection in black-tooted ferrets. Jourtnal of the Attmericanii 5 eterinary Medical Associationi 169, 961-964 FELSENSTEIN, J. (1989) Phylip 3.2 Manual. Berkeley, University of Californlia FORSYTH, M. A. & BARRETT, T. (1995) Evaluation of polymerase chaini reaction for the detection of rinderpest and peste des petits ruminants viruses for

Evaluation of contractile reserve by means of LDD gated SPECT with Tl-201 is safely feasible early after AMI, with incremental value over perfusion assessment alone for myocardial viability detection.

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SHORT COMMUNICATIONS which encode chlamydial heat shock protein 60 (cHSP60) and omp2 protein, respectively. The PCR assays were performed by the method described by Fukushi and Hirai (1994) for groEL and as described by Hartley and others (2001) for omp2. The PCR products were of approximately 1300 bp and 600 bp, as expected. The amplicons were purified and sequenced (GenBank accession numbers AY184288 and AY184289). Comparison by CLUSTAL W of the omp2 sequence of the FEIS-M isolate with that of the FP strain (accession number AF367407) in the GenBank database showed 99-8 per cent similarity. The AluI restriction pattern of the ompA gene of the FEIS-M isolate was found to be identical to that obtained previously from DNA extracted from conjunctival swabs of 12 cats in the same cattery and from 18 cats in two other catteries in Bologna (Di Francesco and others 2003). The nucleotide and predicted amino acid sequences of groEL and omp2 genes of the FEIS-M isolate were also identical to the same regions amplified and sequenced from these conjunctival swabs (GenBank accession numbers AF448139 and AF484215). These results suggest the presence in the area surveyed of only one strain of Cfelis showing a high degree of genomic similarity with other feline chlamydial strains isolated in other countries. In view of the limited geographical area surveyed so far, further investigations of Cfelis isolates from other areas of Italy are necessary to confirm the strain homogeneity.