The Design of Experiments (DOE) and the Analysis of Variance (ANOVA) are used to determine the effect of fuel type, fuel initial load, secondary air inlet and ventilation on thermal efficiency and CO emission of two biomass fire cookstoves during boiling or simmering. Analysis of variance with Fischer's statistical test (F-test) and Newman-Keuls test were applied to establish the influence of the independent parameters on the studied responses. The results of this study are useful to application of charcoal cooks stoves.