No abstract

Here we evaluated the effect of fermented milk supplemented with whey protein (approximately 80 % protein), probiotic (Bifidobacterium animalis subsp. lactis BB12) and pomegranate juice (Punica granatum L.) on the physical performance, intestinal motility and villi structure, inflammatory markers and intestinal microbiota of rats under high-intensity acute exercise. In all, twenty-four Wistar rats were separated into groups: control (Ctrl), supplemented (Supp), exercised (Exe) and exercised and supplemented (Exe+Supp). Rats in the Supp groups received fermented milk during 6 weeks by oral administration. At the end of the supplementation period, the Exe groups were submitted to high-intensity acute exercise on a treadmill. We found that intense acute exercise caused changes in the intestinal villi interspace, changes in the proportion of Lactobacillus species and an increase in Clostridium species, as well as a decrease in intestinal motility. Supplementation increased intestinal motility, and maintained the intestinal villi interspace and the natural microbiota proportions of the exercised rats. Physical performance was not improved by fermented milk supplementation. We conclude that the fermented milk containing whey protein, B. animalis (BB12) and pomegranate juice can re-establish intestinal microbiota and protect the animals from the undesirable effects of intense acute exercise.

The aim of this study was to assess the microbiological profile of leafy vegetables from organic and conventional farming ( = 70). The microbiological parameters analyzed consisted of aerobic mesophilic bacteria, yeasts and molds, coliforms at 30 and 45 ∘ C, and Salmonella ssp. A biochemical identification of the Enterobacteriaceae species was carried out. Some Enterobacteriaceae species were identified in the produce from both farming systems and Hafnia alvei was the most widespread specie observed. Salmonella spp. was not detected in the samples analyzed. The microbial counts for indicator microorganisms were, in general, higher for conventional leaves when compared to those produced by organic farming.

A qualidade microbiológica em lactários é um item que merece toda a atenção e deve ser mantida de forma rigorosa. Considerando a população alvo desses serviços e o ambiente em que estão inseridos, sabe-se que são locais de alto risco para contaminações microbiológicas e as consequências destas podem ser fatais. Assim, o objetivo deste estudo foi verificar a qualidade microbiológica de fórmulas infantis utilizadas para alimentação de crianças internadas na unidade de pediatria de um hospital público do município de Campinas -SP. Microbiological quality of infant formula administered in a public hospital of Campinas -SPThe microbiological quality at lactaries is an item that deserves attention and should be keeping strictly. There are high microbiological risks of contamination at the hospital and the consequence of this can be fatal considering the target population of those services. The objective of this study was to determine the microbiological quality of infant formulas used for feeding infants hospitalized at a pediatric unit of a hospital in the city of Campinas -SP. The infant formulas were evaluated for aerobic mesophilic bacteria, coliforms at 30-35ºC and 45ºC, and

This research evaluated the functional potential of a fermented milk made with Streptococcus thermophilus, a probiotic (Bifidobacterium animalis subsp. lactis) and pomegranate juice. Ferric-reducing antioxidant potential, Trolox equivalent antioxidant capacity, scavenging effect on 1,1-diphenyl-2-picrylhydrazyl free radical, oxygen radical absorbance capacity and Folin-Ciocalteu assays were used to estimate antioxidant activity and phenolic content. Probiotic survival, release and absorption of polyphenols were evaluated using in vitro gastrointestinal digestion. The concentration of polyphenols increased during digestion from 608 mg to 1417 gallic acid equivalents (GAE) /L. About 14% of polyphenols were found on the dialysate portion. The beverage presented high viability of probiotics with high survival rate after digestion (above 7 log cfu/mL).