Cystitis often appears even in absence of bacteria colonization. Trigonitis and interstitial inflammation are the most common morphological features of abacterial cystitis in young and post menopausal women. Arterial obstructive disease and bladder ischemia might play an important role in bladder dysfunction. Activated inflammatory cells produce ROS (radicals of oxygen), NF kB seems involved in ROS synthesis. Clinical studies have indicated that high CO 2 levels can impact upon peripheral tissue, reducing ischaemia, responsible of recurrent inflammation and consequently reducing oxydative phenomena. PRP (platelet-rich plasma) is a volume of fractionated plasma from the patient's own blood that contains platelet concentrate rich of alpha granules. PRP interacts tissue repair mechanisms by placing supra-physiological concentrations of autologous platelets at the site of tissue damage. This study proposes a single PRP transvaginal injection followed by 10 weekly applications of carboxytherapy, using subcutaneous injections of sterile CO2 gas. We have selected 6 Women (50-75 years), affected by recurrent abacterial cystitis with Pain and urge incontinence. All patients showed a subjective sensible reduction of symptoms. After 2 months all patients have neither inflammatory symptoms nor endoscopic evidence of trigonitis. Preliminary qualitative results could encourage the use of carboxytherapy and PRP in treatment of abacterial and interstitial cystitis.

PD (Peyronie's disease) is an acquired disorder of tunica albuginea characterized by the formation of plaques of fibrous tissue often associated to symptoms like ED (erectile dysfunction) and coital pain. The inflammatory process is unknown, even if it is known that Activated inflammatory cells produce many ROS (radical oxygen species), leading to fibroblast proliferation and collagen synthesis. Endothelian dysfunction is the responsible of infiammatory chain reaction in which an inflammatory protein, NF-kB seems involved in ROS synthesis. Conservative treatments (laser, ultrasound, iono/iontophoresis) seem to have poor therapeutic effects in PD. Clinical studies have indicated that altered CO 2 (carbon dioxide) levels can impact upon disease progression. CO 2 levels can be sensed by cells resulting in the initiation of pathophysiologic responses with a sensibile reduction of oxydative phenomena (Bohr/Haldane effects). We have tried carboxytherapy, by using sovrapubic subcutaneous injection of sterile CO2 gas in 20 patients aged from 40 to 65, affected by PD. After the cycle of treatment of 10 weekly applications. We have observed in all patients a subjective reduction of penile deviation, an improvement of quality of erections and a sensible reduction of plaque's dimensions, documented by ultrasound controls and IIEF questionary before and after the end of cycle. We should consider co2 as a powerful antioxidant against endothelian dysfunction and oxydative stress. NF-kB is a target of CO2 antioxidant power. Preliminary qualitative results could encourage an extended use of carboxytherapy in PD treatment.