Air Susu Ibu (ASI) merupakan cairan kehidupan terbaik yang sangatdibutuhkan olehbayi. ASI mengandung berbagai macam zat yang dibutuhkan bagi tumbuh kembanganak. Dalam perkembangannya ibu menyusui kesulitan dalam memenuhi kebutuhan ASI bagi anaknya sehingga dibutuhkan asupan makanan yang membantu memperbanyak produksi ASI,salahsatunya tanaman adas (Foenicullum Vulgare Mill).Tanaman ini mengandung senyawa flavonoid, fenolik, steroid dan komponen minyak atsiri. Dengan adanya senyawa tersebut maka dapat menaikkan produksi ASI dengan melihat kandungan hormon prolaktinnya.Penelitian ini bersifat eksperimental dan bertujuan untuk mengukur pengaruh pemberian ekstrak etanol daun adas terhadap peningkatan kadar hormon prolaktin padahewan uji tikus putih galur Wistar albino pasca melahirkan.Untuk pengujian kadar hormon prolaktin diujikan pada tikus putih yang dibagi 4 (empat) kelompok perlakuan diantaranya: CMC 1% (kontrol); 100; 500; 1000 mg/KgBB. Perlakuan diberikan secara oral pada H + 3 hingga H + 14 setelah melahirkan (postpartum). Hasil dibaca dengan metode Elisa Reader, data yang diperoleh dianalisis dengan uji oneway ANOVA danUji Multiple Comparison (post hoc test) tukeymenggunakan SPSS. Hasil yang diperoleh ekstrak etanol daun adas dapat menaikkan kadar hormon prolaktin pada tikus menyusui dibandingkan dengan kontrol dengan dosis 500 dan 1000 mg/KgBB terdapat perbedaan yang signifikan bermakna dengan nilai p < 0,05.   Kata kunci:  ASI, hormon prolaktin, daun adas     THE INFLUENCE OF FENNEL LEAF ETHANOL EXTRACT PROLACTINE HORMONE LEVELS IN THE BUTINA POST PARTUM RAT   ABSTRACT Mother's Milk (ASI) is the best living fluid that is needed by the baby. Breast milk contains a variety of substances needed for child growth and development. In its development, nursing mothers have difficulty in fulfilling the needs of breast milk for their children, so that food intake is needed to help increase milk production, one of which is the fennel plant (Foenicullum Vulgare Mill). This plant contains flavonoid compounds, phenolics, steroids and essential oil components. With the presence of these compounds, it can increase milk production by looking at the content of the hormone prolactin. This research is experimental and aims to measure the effect of ethanol extract of fennel leaves on increasing levels of the hormone prolactin in animal test rats Wistar albino strain after delivery. To test the level of the hormone prolactin tested in white rats divided into 4 (four) treatment groups including: 1% CMC (control); 100; 500; 1000 mg / kg. The treatment was given orally at H + 3 to H + 14 after delivery (postpartum). The results were read by the Elisa Reader method, the data obtained were analyzed by the ANAVA one way test and the Tuckey Multiple Comparison Test (post hoc test) using SPSS. The results obtained ethanol extract of fennel leaves can increase levels of the hormone prolactin in lactating mice compared with controls with doses of 500 and 1000 mg / KgBB there is a significant difference with p value <0.05.   Keywords: ASI, prolactin hormone, fennel leaf

Free radicals can cause several diseases. Antioxidants are the compounds that can reduce free radicals in the human body. Kaffir lime leaves contain phenolic and flavonoids compounds which have antioxidant activity. This research aimed to evaluate the antioxidant activity and to determine total phenolic contents of fraction n-hexane and chloroform from kaffir lime leaves. The antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The total phenolic content was determined by the spectrophotometric method. The results showed that the antioxidant activity of chloroform fraction (IC50: 302.91 ± 0.28 µg / mL) was higher than the n-hexane fraction (IC 50 : 714,25±1,97 µg /mL),but the antioxidant activity was lower than vitamin C (IC 50 : 3,28 ±0,12 µg /mL). The total phenolic content of chloroform fraction (4.73±0,33% w/w EAG) was higher than the n-hexane fraction (3,18 ± 0,31% w/w EAG).

Free radicals can cause several diseases. Antioxidants are the compounds that can reduce free radicals in the human body. Kaffir lime leaves contain phenolic and flavonoids compounds which have antioxidant activity. This research aimed to evaluate the antioxidant activity and to determine total phenolic contents of fraction n-hexane and chloroform from kaffir lime leaves. The antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The total phenolic content was determined by the spectrophotometric method. The results showed that the antioxidant activity of chloroform fraction (IC50: 302.91 ± 0.28 µg / mL) was higher than the n-hexane fraction (IC 50 : 714,25±1,97 µg /mL),but the antioxidant activity was lower than vitamin C (IC 50 : 3,28 ±0,12 µg /mL). The total phenolic content of chloroform fraction (4.73±0,33% w/w EAG) was higher than the n-hexane fraction (3,18 ± 0,31% w/w EAG).

<p class="abstract">Produk kosmetik yang mengandung bahan alam sebagai antioksidan semakin banyak digunakan oleh masyarakat. Salah satunya produk kosmetik Krim “X” yang mengandung ekstrak temulawak. Penelitian ini dilakukan untuk mengetahui aktivitas antioksidan Krim”X”. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Hasil analisia kualitatif menunjukkan bahwa Krim “X” mempunyai aktivitas antioksidan ditandai dengan memudarnya warna ungu pada larutan DPPH 0,4 mM menjadi warna kuning. Sedangkan secara Analisa kuantitatif menunjukkan bahwa krim “X” mempunyai aktivitas antioksidan sangat lemah dengan nilai IC₅₀ sebesar 969,65 ± 23,27 ppm.</p>

ABSTRAKAntioksidan merupakan senyawa yang dapat menyeimbangkan ROS (reactive oxygen species) yang dapat menyebabkan penuaan kulit. Tubuh manusia secara alamiah dapat menghasilkan antioksidan akan tetapi jumlahnya terbatas sehingga perlu antioksidan eksogen. Daun jeruk purut merupakan tanaman yang mengandung senyawa fenolik dan flavonoid yang berpotensi sebagai antioksidan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan fraksi n-heksan dan fraksi kloroform ekstrak etanol daun jeruk purut dengan metode FRAP (Ferric Reducing Antioxidant Power). Metode ekstraksi menggunakan maserasi dengan pelarut etanol 96%. Fraksinasi menggunakan metode partisi dilanjutkan uji aktivitas antioksidan dengan metode FRAP secara spektrofotometri UV-Vis. Hasil penelitian menunjukkan bahwa fraksi n-heksan dan fraksi kloroform memiliki aktivitas antioksidan dengan nilai EC50 masing-masing sebesar (9870,36 ± 1,55) µg/mL dan (9713,30 ± 0,70) µg/mL. Berdasarkan hasil penelitian dapat disimpulkan bahwa fraksi n-heksan dan fraksi kloroform ekstrak etanol daun jeruk purut mempunyai aktivitas antioksidan yang sangat lemah.Kata kunci : FRAP; antioksidan; fraksi n-heksan; fraksi kloroform; daun jeruk purut.ABSTRACT Antioxidants are compounds that can balance ROS (reactive oxygen species) which can cause skin aging. The human body can naturally produce antioxidants but the amount is limited so it needs exogenous antioxidants. Kaffir lime leaves are plants that contain phenolic and flavonoid compounds that have the potential as antioxidants. This study aims to determine the antioxidant activity of the n-hexane fraction and the chloroform fraction of ethanol extract of kaffir lime leaves using the FRAP (Ferric Reducing Antioxidant Power) method. The extraction method uses maceration with 96% ethanol as solvent. Fractionation using partition method followed by antioxidant activity test using FRAP method by UV-Vis spectrophotometry. The results showed that the n-hexane and the chloroform fractions had antioxidant activity with EC50 values of (9870.36 ± 1.55) g/mL and (9713.30 ± 0.70) g/mL. Based on the results of the study, it can be concluded that the n-hexane fraction and the chloroform fraction of the ethanol extract of kaffir lime leaves had very weak antioxidant activity. Keywords : FRAP; antioxidants; n-hexane fraction; chloroform fraction; lime leaves.